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Custom Vector Construction> | The C. sativa L. O-methyltransferase open reading frames were synthesized by GenScript. These cDNAs for CsOMT6, 12 and 21 were amplified by PCR using the KOD Hot Start DNA polymerase (Novagen) and then ligated between the NdeI/AseI and HindIII sites of the pET28b vector system (Novagen) which introduces an N-terminal 6 x His tag to each coding sequence. | Get A Quote |
In addition to the psychoactive constituents that are typically associated with Cannabis sativa L., there exist numerous other specialized metabolites in this plant that are believed to contribute to its medicinal versatility. This study focused on two such compounds, known as cannflavin A and cannflavin B. These prenylated flavonoids specifically accumulate in C. sativa and are known to exhibit potent anti-inflammatory activity in various animal cell models. However, almost nothing is known about their biosynthesis. Using a combination of phylogenomic and biochemical approaches, an aromatic prenyltransferase from C. sativa (CsPT3) was identified that catalyzes the regiospecific addition of either geran... More