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Proteins, Expression, Isolation and Analysis> | Samples were run on a 4–12% gradient TrisMOPS-Gel (GenScript) and transferred to polyvinylidene fluoride membranes. An anti-S2 MERS-CoV S polyclonal antibody (1:2000 dilution; Thermo_Fisher; Cat#PA5-81788) and an HRP-conjugated goat anti-rabbit secondary antibody (1:500 dilution; | Get A Quote |
Most neutralizing antibodies against Middle East respiratory syndrome coronavirus (MERS-CoV) target the receptor-binding domain (RBD) of the spike glycoprotein and block its binding to the cellular receptor dipeptidyl peptidase 4 (DPP4). The epitopes and mechanisms of mAbs targeting non-RBD regions have not been well characterized yet. Here we report the monoclonal antibody 7D10 that binds to the N-terminal domain (NTD) of the spike glycoprotein and inhibits the cell entry of MERS-CoV with high potency. Structure determination and mutagenesis experiments reveal the epitope and critical residues on the NTD for 7D10 binding and neutralization. Further experiments indicate that the neutralization by 7D10 is not so... More