Products/Services Used | Details | Operation |
---|---|---|
Bacterial Expression System> | The coding sequences of human PI3K p85α (catalog no. OHu21143D; GenScript) corresponding to 1–315 aa and various human CD2AP constructs (FL-CD2AP 1–639 aa; CD2AP 1–335 aa, 1–475 aa, 336–605 aa; GFP-1-475 aa; and GFP-1- 335 aa) were amplified by PCR (iProof) and cloned in-frame into the bacterial expression vector pET30a+ (EMD) with 6-His tag at the N-terminus for expression in BL21 cells (NEB). | Get A Quote |
Lysophosphatidic acid (LPA) is a bioactive lipid, which plays an indispensable role in various physiological and pathological processes. Moreover, an elevated level of LPA has been observed in malignancies of different origins and implicated in their progression via modulation of proliferation, apoptosis, invasion and metastasis. Interestingly, few recent reports suggest a pivotal role of LPA-modulated metabolism in oncogenesis of ovarian cancer. However, little is understood regarding the role of LPA in the development and progression of T cell malignancies, which are considered as one of the most challenging neoplasms for clinical management. Additionally, mechanisms underlying the LPA-dependent modulation of... More