The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Targeting specific loci in the genome for mutagenesis would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.
The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Targeting specific loci in the genome for mutagenesis would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.
