Products/Services Used | Details | Operation |
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Molecular Biology Reagents> | … The N2MPP was synthesized as gBlock Gene (GenScript), PCR-amplified, and cloned into the pACEBac2 vector (Geneva Biotech) under the control of the AcMNPV p10 promoter. … | Get A Quote |
Vaccination against influenza virus can reduce the risk of influenza by 40% to 60%, they rely on the production of neutralizing antibodies specific to influenza hemagglutinin (HA) ignoring the neuraminidase (NA) as an important surface target. Vaccination with standardized NA concentration may offer broader and longer-lasting protection against influenza infection. In this regard, we aimed to compare the potency of a NA displayed on the surface of a VLP with a soluble NA. The baculovirus expression system (BEVS) and the novel virus-free Tnms42 insect cell line were used to express N2 NA on gag-based VLPs. To produce VLP immunogens with high levels of purity and concentration, a two-step chromatography purificat... More