![munber 1 munber 1](/gsimgs/subject/case-study-step-num1.png)
Antigen Analysis: Human 4-1BB ECD Alignment
ECD Domain | Human | Cyno | Mouse | Rabbit |
---|---|---|---|---|
Human Homology | 100% | 95% | 57% | 68% |
![munber 2 munber 2](/gsimgs/subject/case-study-step-num2.png)
High positive hits screening with Beacon
Assay (SA) beads assay
![Assay (SA) beads assay](/gsimgs/subject/assay-beads-assay.png)
Valid Nano-well: 7560; Positive cell : 932
Positive%: 12.3%
Cell-based assay
![Cell-based assay](/gsimgs/subject/cell-based-assay.png)
Valid Nano-well: 7560; Positive cell : 112
Positive%: 1.48%
![munber 3 munber 3](/gsimgs/subject/case-study-step-num3.png)
NGS sequencing
![munber 4 munber 4](/gsimgs/subject/case-study-step-num4.png)
Validation of recombinant antibodies with ELISA & FACS
![case step 4 icon1 case step 4 icon1](/gsimgs/subject/case-step4-icon1.png)
![case step 4 arrow case step 4 arrow](/gsimgs/subject/case-step4-arrow.png)
![case step 4 icon2 case step 4 icon2](/gsimgs/subject/case-step4-icon2.png)
![case step 4 arrow case step 4 arrow](/gsimgs/subject/case-step4-arrow.png)
![case step 4 icon3 case step 4 icon3](/gsimgs/subject/case-step4-icon3.png)
![case step 4 arrow case step 4 arrow](/gsimgs/subject/case-step4-arrow.png)
![case step 4 icon4 case step 4 icon4](/gsimgs/subject/case-step4-icon4.png)
FACS, Median Fluorescence Intensity (MFI) +/-
![FACS, Median Fluorescence Intensity (MFI) +/-](/gsimgs/subject/facs-median-fluorescence-intensity.png)
![munber 5 munber 5](/gsimgs/subject/case-study-step-num5.png)
Affinity analysis of 4-1BB recombinant antibodies
![KD (M) = 9.701E-08](/gsimgs/subject/case-study-sample-id-clone17.png)
KD (M) = 9.701E-08
![KD (M) = 7.069E-10](/gsimgs/subject/case-study-sample-id-clone-2-66.png)
KD (M) = 7.069E-10