| Specificity |
THE™
His Tag Antibody [HRP], mAb, Mouse recognizes His tags localized at C-terminal,
N-terminal, and internal region of fusion proteins.
|
| Host Species |
Mouse |
| Immunogen |
A synthetic peptide HHHHHH coupled to KLH |
| Conjugate |
Horseradish peroxidase (HRP) |
Working
concentrations for specific applications should be determined by the
investigator. The appropriate concentrations may be affected by secondary
antibody affinity, antigen concentration, the sensitivity of the method of
detection, temperature, the length of the incubations, and other factors. The
suitability of this antibody for applications other than those listed below has
not been determined. The following concentration ranges are recommended
starting points for this product.
|
| Application |
Recommended Usage |
| ELISA |
0.05-0.5 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized
with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
|
| Reconstitution |
Reconstitute
the lyophilized powder with deionized water (or equivalent) to a final
concentration of 0.5 mg/mL.
|
| Storage Instructions |
The
lyophilized product remains stable for up to 1 year at -20°C from date of
receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For
long term storage, aliquot and store at -20°C or below. Upon receiving, spin
the vial down to place the lyophilized antibody on the bottom of the vial.
Reconstitute the antibody as mentioned above. Prepare single use aliquots (no
less than 10µl) in amber vials and freeze it immediately at -20oC. The
frozen aliquots should be thawed once with any remainder kept at 4oC.
|
| Purification |
Purified
by Protein A affinity chromatograph.
|
| Isotype |
Mouse IgG1,κ |
| Clonality |
Monoclonal |
| Clone ID |
6G2A9 |
![THE™ His Tag Antibody [HRP], MAb, Mouse](/product/documents/down?doc_name=f8dcb9be-b4bf-4206-a699-8c10661e276b.jpg&file=scm_files/productFile_notes/2023/11/20/24b0e22a-b83d-47b3-8db1-c782fb508ced.jpg "THE™ His Tag Antibody [HRP], MAb, Mouse")
Figure 1. A recombinant His-tagged protein was detected by Western blotting using THE™ His Tag Antibody [HRP], mAb, Mouse (A00612) at 1 µg/mL concentration.
The signal was developed with LumiSensor™ HRP Substrate Kit (GenScript, L00221, discontinued).
| Target Background |
GenScript’s
THE™ His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THE™ Anti-His
Monoclonal Antibody (Mouse) (A00186) purified by protein A affinity chromatograph.
This product is suitable for detecting the expression level of His-tagged
proteins various immunoassays.
|
| Synonyms |
His-tag
Antibody [HRP],mAb, Mouse
|
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
