| Specificity |
This Antibody recognizes Strep-tag II tagged fusion proteins. |
| Host Species |
Rabbit |
| Immunogen |
Strep-tag II epitope peptide NWSHPQFEK conjugated to KLH. |
| Conjugate |
Horseradish peroxidase (HRP) |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.5-1.0 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
![NWSHPQFEK Antibody [HRP], PAb, Rabbit](/product/documents/down?doc_name=9823_20090524212741.JPG&file=scm_files/productFile_notes/2023/11/20/dc29bad0-df1b-4662-a81a-115d948e8dff.JPG "NWSHPQFEK Antibody [HRP], PAb, Rabbit")
Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody [HRP], pAb, Rabbit (GenScript, A00875, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 60 kD
Observed Size: 60 kD
| Target Background |
Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered.The Rabbit anti NWSHPQFEK polyclonal antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase.The antibody is suitable for detecting fusion proteins that contain NWSHPQFEK. |
| Synonyms |
Rabbit anti NWSHPQFEK [HRP] pAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
