| Specificity |
Rabbit Anti-Akt (Phospho-Ser473) detects endogenous human, mouse, and rat Akt1, Akt2 and Akt3, phosphorylated at serine 473, serine 474, and serine 472, respectively. |
| Host Species |
Rabbit |
| Immunogen |
Synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S) |
| Species Reactivity |
Human, mouse, rat |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.5-2.0 µg/ml |
| Other applications |
User-optimized |
|
| Form |
Liquid |
| Storage Buffer |
1×PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide |
| Concentration |
0.5 mg/ml |
| Storage Instructions |
The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
| Target Background |
Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes.Akt is activated following its phosphorylation at two regulatory residues.Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation.Akt activity is augmented approximately ten fold by phosphorylation at the serine on the hydrophobic motif by PDK2.Phosphorylation of Thr308 and Ser473 activates Akt α.Phosphorylation at Thr309 and Ser474 on Akt β1 and β2, and on Thr305 on Akt γ results in their activation.Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including bad, forkhead transcription factors, c-Raf and caspase-9.The activation of Akt is negatively regulated by PTEN, a PIP3-specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase.GenScript Rabbit Anti-Akt (Phospho-Ser473) Polyclonal Antibody is developed in rabbit using a synthetic phosphopeptide corresponding to residues surrounding S473 of human, rat, and mouse Akt α, also known as Protein Kinase B alpha (PKBα), Akt1, and RACα.In addition, this phosphopeptide is identical to the sequence surrounding S474 of human, rat, and mouse Akt β (Akt2) and S472 of human and mouse Akt γ (Akt3). |
| Synonyms |
Rabbit Anti-Akt (Phospho-Ser473) pAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
