| Specificity |
THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins. |
| Host Species |
Mouse |
| Immunogen |
A synthetic peptide (DYKDDDDK) coupled to KLH |
| Conjugate |
Biotin |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2b,κ |
| Clonality |
Monoclonal |
| Clone ID |
5A8E5 |
![THE™ DYKDDDDK Tag Antibody [Biotin], MAb, Mouse](/product/documents/down?doc_name=10211_20090721015900.JPG&file=scm_files/productFile_notes/2023/11/20/0b1c8ea2-8296-40b2-9abc-bf93b5cd5681.JPG "THE™ DYKDDDDK Tag Antibody [Biotin], MAb, Mouse")
Loading: N-terminal DYKDDDDK-fusion protein (400 ng, 100 ng, 25 ng)
Primary antibody: 1 μg/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Biotin) (GenScript, Cat. No. A01429, Lot No. A213902)
Secondary antibody: Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091)
| Target Background |
GenScript THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THE™ DYKDDDDK Tag Antibody, mAb, Mouse (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins. |
| Synonyms |
anti Flag-tag mAb; anti DYKDDDDK-tag mAb |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
