| Specificity |
The product is specific for human CTLA4 (UniProt Accession: P16410). This antibody blocks human CTLA4 ligand binding in ELISA assay and its signaling in cell based functional assay. |
| Host Species |
Mouse |
| Immunogen |
Recombinant human CTLA4-Fc (Z03373) |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.01-0.1 µg/ml |
| Competitive ELISA |
10-50 µg/ml |
| Flow Cytometry |
2-10 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG1,κ |
| Clonality |
Monoclonal |
| Clone ID |
CT.E8 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
| Target Background |
CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. CTLA-4 is recruited from intracellular vesicles to the immunological synapse beginning 1-2 days after T cell activation. It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation. |
| Synonyms |
Mouse monoclonal to cytotoxic T-lymphocyte associated protein 4/CD/ GSE/ GRD4/ ALPS5/ CD152/ CTLA-4/ IDDM12/ CELIAC3 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
