| Specificity |
The product is specific for human TIM3 (UniProt Accession: Q8TDQ0). This antibody specifically recognizes human TIM3 recombinant protein in ELISA assay and human TIM3-expressing stable cell line in FACS assay. |
| Host Species |
Mouse |
| Immunogen |
Recombinant human TIM3-Fc (2365-TM) |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.01-0.1 µg/ml |
| Flow Cytometry |
2-10 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG1,κ |
| Clonality |
Monoclonal |
| Clone ID |
TI.B2 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
| Target Background |
TIM-3 (T cell immunoglobulin and mucin domain-3), is a member of the TIM family of immune regulating molecules. Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity.GenScript Human TIM3 Antibody (TI.B2), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant human TIM3-Fc (2365-TM). |
| Synonyms |
Mouse monoclonal to T-Cell Immunoglobulin Mucin Receptor 3/HAVCR2/ CD366/ KIM-3/ TIMD3/ Tim-3/ TIMD-3/ HAVcr-2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
