| Specificity |
The product is specific for Staphylocoagulase. This antibody binds with recombinant Staphylocoagulase protein in ELISA and western blot. |
| Host Species |
Mouse |
| Immunogen |
Recombinant Staphylocoagulase |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA detection |
0.01-1 µg/ml |
| Western Blot |
1 µg/ml |
| Other applications |
User-optimized |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2b,κ |
| Clonality |
Monoclonal |
| Clone ID |
14A10 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
| Target Background |
Staphylocoagulase (SC) is a protein secreted by most strains of Staphylococcus aureus, a potent human pathogenic bacterium. SC causes blood clotting by direct activation of the thrombin precursor, prothrombin (ProT). This occurs through a non-proteolytic, cofactor-induced mechanism of activation called “molecular sexuality”. Staphylocoagulase is a potent nonproteolytic prothrombin (ProT) activator and the prototype of a newly established zymogen activator and adhesion protein family. SC-bound ProT efficiently clots fibrinogen, thus bypassing the physiological blood coagulation pathway. |
| Synonyms |
Mouse monoclonal to Staphylocoagulase |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
