| Specificity |
The product is specific for human CD27 |
| Host Species |
Mouse |
| Immunogen |
Recombinant CD27 protein |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.01-0.05 µg/ml |
| Flow Cytometry |
1- 5 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2b |
| Clonality |
Monoclonal |
| Clone ID |
16G7 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
| Target Background |
CD27, also known as S152 and T14, is a member of the tumor necrosis factor receptor family. It is expressed on memory B cells, a fraction of plasma cells, naive and memory T cells and NK cells. CD27 binds to ligand CD70, and plays an important role in regulating B-cell activation and immunoglobulin synthesis. It is considered as a useful marker to distinguish certain T and B cell subsets from each other. |
| Synonyms |
Mouse monoclonal to Human CD27/S152/T14/TNFRSF7/Tp55 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
