| Specificity |
The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody GenScript, A01985-40, MonoRab Anti-Atezolizumab Antibody (43H11), mAb, Rabbit. |
| Host Species |
Rabbit |
| Immunogen |
Atezolizumab |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Sandwich ELISA |
Capture: 0.01-0.1 µg/ml |
| Competitive ELISA |
10-50 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
55D8 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
ELISA binding of MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8) (GenScript, A01984-40) with Atezolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Atezolizumab, 1 µg/ml.
MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8) (GenScript, A01984-40) dilutions start from 1,000 ng/ml.
EC₅₀= 2.446 ng/ml.
Standard curve of Atezolizumab Sandwich ELISA. The Atezolizumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit (GenScript, A01984-40) and MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11) (Genscript, A01985-40) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11) (GenScript, A01985-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.The sensitivity of detecting Atezolizumab is up to 156 pg/ml.
MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit (GenScript, A01984-40) blocks Atezolizumab binding with Human PD-L1 recombinant protein (Z03371, PD-L1 Fc Chimera, Human).
Coating antigen: Atezolizumab, 1 μg/ml.
PD-L1-Fc-biotin final concentration: 10 ng/ml.
Anti-Atezolizumab antibody dilutions start from 10 μg/ml.
IC₅₀= 0.39 μg/ml
| Target Background |
Atezolizumab is a fully humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of metastatic non-small cell lung cancer (NSCLC). It binds to the protein programmed cell death-ligand 1 (PD-L1) on several tumors. Atezolizumab has ability to block the binding of PD-L1 with PD-1 and CD80 receptors (B7-1Rs), which has a positive effect on improving an anti-tumor response.GenScript MonoRab™ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Atezolizumab. |
| Synonyms |
Rabbit monoclonal to Tecentriq |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
