![His Tag Antibody (C157), MAb, Mouse His Tag Antibody (C157), MAb, Mouse](/product/documents/down?doc_name=a02003_elisa.jpg&file=scm_files/productFile_notes/2019/02/20/4458a8e7a55c21002dd879465535fc3f.jpg)
ELISA binding of His tag Antibody (C157) (GenScript, A02003) with His-tag fusion protein.
Coating antigen: His-tagged protein, 1 μg/ml.
Antibody dilutions: Start from 1,000 ng/ml.
EC₅₀= 4.694 ng/ml.
![His Tag Antibody (C157), MAb, Mouse His Tag Antibody (C157), MAb, Mouse](/product/documents/down?doc_name=a02003_ip.jpg&file=scm_files/productFile_notes/2019/02/20/ca736ce2471ecce57085bc54792c6e38.jpg)
Immunoprecipitation (IP) assay of His tag Antibody (C157)(GenScript, A02003) with cell lysate transfected with His-tag fusion protein.
Loading:
Lane 1: Cell lysate
Lane 2: IP with mouse IgG; 1:200
Lane 3: IP with His tag Antibody (C157); 1:200
Lane M: Protein Marker
Primary Antibody:
MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), mAb,Rabbit (GenScript, A01857-40, 0.5 μg/ml);
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211),0.125 μg/ml
![His Tag Antibody (C157), MAb, Mouse His Tag Antibody (C157), MAb, Mouse](/product/documents/down?doc_name=a02003_wb.jpg&file=scm_files/productFile_notes/2019/02/20/ca7b09d786a02377143bada81c571e76.jpg)
Western blot analysis of His tag Antibody (C157) (GenScript, A02003) with His tag fusion protein Loading:
Lane M: Protein Marker;
Lane 1: 100 ng His tag fusion protein
Lane 2: 50 ng His tag fusion protein
Lane 3: 25 ng His tag fusion protein
Lane 4: 10 ng His tag fusion protein
Primary Antibody:
His tag Antibody (C157) (GenScript, A02003); 1:1000
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) ;0.125 μg/ml
His tag Antibody (C157), mAb, Mouse
A02003 | |
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¥1200 | |
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