ELISA binding of His tag Antibody (C157) (GenScript, A02003) with His-tag fusion protein.
Coating antigen: His-tagged protein, 1 μg/ml.
Antibody dilutions: Start from 1,000 ng/ml.
EC₅₀= 4.694 ng/ml.

Immunoprecipitation (IP) assay of His tag Antibody (C157)(GenScript, A02003) with cell lysate transfected with His-tag fusion protein.
Loading:
Lane 1: Cell lysate
Lane 2: IP with mouse IgG; 1:200
Lane 3: IP with His tag Antibody (C157); 1:200
Lane M: Protein Marker
Primary Antibody:
MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), mAb,Rabbit (GenScript, A01857-40, 0.5 μg/ml);
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211),0.125 μg/ml

Western blot analysis of His tag Antibody (C157) (GenScript, A02003) with His tag fusion protein Loading:
Lane M: Protein Marker;
Lane 1: 100 ng His tag fusion protein
Lane 2: 50 ng His tag fusion protein
Lane 3: 25 ng His tag fusion protein
Lane 4: 10 ng His tag fusion protein
Primary Antibody:
His tag Antibody (C157) (GenScript, A02003); 1:1000
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) ;0.125 μg/ml