| Specificity |
The product is specific for SARS-CoV-2 Spike Protein S1 subunit and its RBD domain. |
| Host Species |
Rabbit |
| Immunogen |
Recombinant SARS-CoV-2 Spike protein fragment |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.01-0.1 µg/ml |
| Western Blot |
1 µg/ml |
| Flow Cytometry |
10 µg/ml |
| Surrogate Virus Neutralization Test (sVNT) |
0.2-1 µg/ml |
|
| Form |
Liquid |
| Storage Buffer |
0.2 μm filtered solution in PBS, pH 7.4 |
| Concentration |
1 mg/ml |
| Storage Instructions |
Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
4G6 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
Western Blot analysis of MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit (GenScript, A02053) with SARS-CoV-2 RBD.
Lane 1: Negative cell supernatant of RBD ,10 μl
Lane 2: Positive cell supernatant of RBD , 10 μl
Lane 3: SARS-CoV-2 RBD Protein , 20 ng
Primary Antibody:
MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit , 1 μg/ml
Secondary Antibody:
Anti-Rabbit IgG (H&L) Goat Antibody Peroxidase Conjugated
ELISA binding of MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit (GenScript, A02053) with recombinant SARS-CoV-2 Spike Protein S1 RBD(GenScript, Z03483) and SARS-CoV RBD.
| Target Background |
SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). SARS-CoV-2 contains glycosylated spike (S) protein, which is composed of S1 subunit and S2 subunit. The S1 contains a receptor-binding domain (RBD) that can bind to ACE2 receptor on target cells. |
| Synonyms |
2019-nCoV Neutralizing Antibody, mAb, Rabbit |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
