| Specificity |
The product is specific for Human Claudin 18.2. |
| Host Species |
Rabbit |
| Immunogen |
Recombinant Human Claudin 18.2 Protein and DNA |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
1/5,000 - 1/10,000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF) |
1/100 - 1/1,000 |
| Immunohistochemistry (IHC) |
1/200 - 1/800 |
| Flow Cytometry |
1/100 - 1/1,000 |
|
| Form |
Liquid |
| Storage Buffer |
Supplied in PBS, pH 7.4, containing 0.02% ProClin300 |
| Concentration |
1.5 mg/mL |
| Storage Instructions |
The product remains stable up to 1 year at -20 °C from date of receipt. Avoid repeated freezing and thawing cycles. |
| Purification |
Purified by Protein A affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
126C1 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
Figure 1. The specificity of MonoRab™ Claudin 18.2 Antibody (126C1), mAb, Rabbit (GenScript, A02180) for human Claudin 18.2 was analyzed in an immunocytochemistry/immunofluorescence analysis of Claudin 18.2 transfected Vero cells and Claudin 18.1 transfected CHO-K1 cells.
Figure 2. Immunohistochemical analysis of tumor tissue formed by subcutaneous injection of hCLDN18.2 overexpressed 293T cell spheroid into mice using MonoRab™ Claudin 18.2 Antibody (126C1), mAb, Rabbit (GenScript, A02180). MonoRab™ Claudin 18.2 Antibody (126C1), mAb, Rabbit (GenScript, A02180) has membrane staining on tissue sections.
Figure 3. Flow cytometry analysis of Vero cells transiently transfected with expression vectors encoding human Claudin 18.2 (Red) and Vero negative control cell (Blue) binding with MonoRab™ Claudin 18.2 Antibody (126C1), mAb, Rabbit (GenScript, A02180).
| Target Background |
Claudin-18 is a protein that in humans is encoded by the CLDN18 gene. CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. The expression of isoform A2 (CLDN18.2) is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level). However, CLDN18.2 is highly expressed in certain primary malignant tumors, such as gastric cancer (GC), breast cancer, colon cancer and liver cancer. CLDN18.2 is expressed in 50 - 70% of primary gastric adenocarcinomas and their metastases, thus it is considered a promising candidate for targeted gastric cancer therapy. Therapeutic antibodies against Claudin 18.2 have great therapeutic potential and very low toxicity. |
| Synonyms |
Claudin-18; CLDN18; SFTA5; SFTPJ; claudin 18 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
