| Specificity |
The product is specific to Biotin and biotinylated antibody, protein, peptides, oligonucleotides, or solid matrices. |
| Host Species |
Rabbit |
| Conjugate |
Horseradish peroxidase (HRP) |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
1:1000-1:5000 |
| Western Blot |
1:1000-1:5000 |
|
| Form |
Liquid |
| Storage Buffer |
Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.01% thimerosal and 50% glycerol. |
| Concentration |
0.5 mg/ml |
| Storage Instructions |
Store at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification |
Protein A/G affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
53C8 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
| Target Background |
Biotin is widely conjugated to proteins and antibodies for biochemical assays. Avidin (streptavidin)-biotin system is commonly used for many immunoassays such as ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, and Immunohistochemistry. Anti-Biotin mAb is a better alternative to avidins to minimize background and maximize signal intensity. |
| Synonyms |
Biotin Antibody [HRP]; anti-Biotin Antibody [HRP]; HRP conjugated Biotin monoclonal antibody; MonoRab™ anti-Biotin Antibody [HRP] |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
