BLI (Biolayer interferometry) binding affinity measurements of MonoRab™ Anti-Ixekizumab Antibody (29B12), mAb, Rabbit (GenScript, A02265) to Ixekizumab.
Ixekizumab captured on HFC (Anti-Human IgG Fc) Probes can bind MonoRab™ Anti-Ixekizumab Antibody (29B12), mAb, Rabbit with a dissociation constant (KD) of less than 1 pM.

Anti-Ixekizumab Antibody (19F6)) or (29B12) inhibits the binding of Ixekizumab to IL-17A. Coating antigen: Ixekizumab 30 µg/ml.
IL-17A (GenScript, Z03228) final concentration: 20 ng/ml.
Anti-Ixekizumab antibody dilutions start from 30 μg/ml.
MonoRab™ Anti-Ixekizumab Antibody (19F6), mAb, Rabbit (GenScript, A02264) demonstrated inhibitory properties with an IC₅₀ value of 0.32 µg/ml and MonoRab™ Anti-Ixekizumab Antibody (29B12), mAb, Rabbit (GenScript, A02265) demonstrated inhibitory properties with an IC₅₀ value of 1.11 µg/ml

Standard curve of Ixekizumab sandwich ELISA by A02264 (19F6) and A02265 (29B12).
In this ELISA assay, MonoRab™ Anti-Ixekizumab Antibody (19F6), mAb, Rabbit (GenScript, A02264) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Ixekizumab Antibody (29B12), mAb, Rabbit (GenScript, A02265) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/ml.
In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99921. The typicaldynamic range of the assay is 1-64 ng/mL and its sensitivity of detecting Ixekizumab is up to 1 ng/ml.

MRD analysis of the detection of Ixekizumab in biological matrix.

The MRD is the minimum dilution necessary for the detection of Ixekizumab in biological matrix with least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggestedthat MRD was as 1:10.
In this ELISA assay, MonoRab™ Anti-Ixekizumab Antibody (19F6), mAb, Rabbit (GenScript, A02264) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Ixekizumab Antibody (29B12), mAb, Rabbit (GenScript, A02265) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/ml.

Specificity analysis of the detection of Ixekizumab.

Ixekizumab standard samples at 8 concentrations were spiked with 100 ng/mL of human IgG4. The test result demonstrated that the concentration of human IgG4 did not interfere with the detection of Ixekizumab.
In this ELISA assay, MonoRab™ Anti-Ixekizumab Antibody (19F6), mAb, Rabbit (GenScript, A02264) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Ixekizumab Antibody (29B12), mAb, Rabbit (GenScript, A02265) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/ml.