BLI (Biolayer interferometry) binding affinity measurements of MonoRab™ Anti-OKT3/Muromonab Antibody (40D3), mAb, Rabbit (GenScript, A02312) to OKT3 monoclonal antibody.
OKT3 monoclonal antibody captured on MFC (Anti-Mouse IgG Fc) Probes can bind MonoRab™ Anti-OKT3/Muromonab Antibody (40D3), mAb, Rabbit with a dissociation constant (KD) of 1.30E-10M.

Standard Curve of OKT3 monoclonal antibody Sandwich ELISA by A02312 (40D3) and A02313 (9E1)
In this Sandwich ELISA assay, MonoRab™ Anti-OKT3/Muromonab Antibody (40D3), mAb, Rabbit (GenScript, A02312) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-OKT3/Muromonab Antibody (9E1), mAb, Rabbit (GenScript, A02313) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL. GenScript can provide customized conjugation service for this product per customer's request.
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99998. The typicaldynamic range of the assay is 0.08 - 5 ng/mL.

MRD Analysis of the Detection of OKT3 monoclonal antibody in Biological Matrix.
The MRD is the minimum dilution necessary for the detection of OKT3 monoclonal antibody in biological matrix with the least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggested that MRD was as 1:10.
In this ELISA assay, MonoRab™ Anti-OKT3/Muromonab Antibody (40D3), mAb, Rabbit (GenScript, A02312) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-OKT3/Muromonab Antibody (9E1), mAb, Rabbit (GenScript, A02313) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL.

Specificity analysis of the detection of OKT3 monoclonal antibody.
Samples of OKT3 monoclonal antibody at 8 concentrations were spiked with 100 ng/mL of human IgG or Mouse IgG2a. The test results demonstrated that the high concentration of human IgG or Mouse IgG2a did not interfere with the detection of OKT3 monoclonal antibody.
In this ELISA assay, MonoRab™ Anti-OKT3/Muromonab Antibody (40D3), mAb, Rabbit (GenScript, A02312) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-OKT3/Muromonab Antibody (9E1), mAb, Rabbit (GenScript, A02313) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL.

Anti-OKT3/Muromonab Antibodies inhibit the binding of OKT3 monoclonal antibody to Human CD3.

In this assay, the coating antigen was Human CD3 epsilon &CD3 delta Heterodimer Protein at a concentration of 1 μg/mL. Then both OKT3 and either the Anti-OKT3 monoclonal antibody Antibody (40D3) or (9E1) were added to the plate. The concentration of OKT3 was 50 ng/mL, and the Anti-OKT3 monoclonal antibody Antibody (40D3) or (9E1) was diluted in a 3-fold gradient from a concentration of 30 μg/mL. The secondary antibody was anti-Mouse IgG Fc Secondary Antibody (HRP).
MonoRab™ Anti-OKT3/Muromonab Antibody (40D3), mAb, Rabbit (GenScript, A02312) exhibited non-inhibitory properties, whereas MonoRab™ Anti-OKT3/Muromonab Antibody (9E1), mAb, Rabbit (GenScript, A02313) demonstrated significant inhibitory properties.