GenCRISPR™ NLS-wtSpCas9, his-tag

该产品目前处于待上市状态。

This newly wtSpCas9 is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with an nuclear localization signal (NLS) and his-tag.

产品编号	RC00001
规格	大单询价

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Description

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9.

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

应用指导

Genome editing with CRISPR T-Cells, Stem Cells, etc.

质控参数

Appearance	Clear liquid
Purity	≥ 95.0% by SEC-HPLC, ≥ 95.0% by Bis-Tris PAGE
Quality	Antibiotic-free and animal-free production
Concentration	9.5-12.5 mg/mL
Bioactivity	cleavage activity>85%
Residual RNase	Negative
Endotoxin Level	≤ 10.0EU/mg

产品属性

Synonyms	CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9
Source	This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification.
Species	Streptococcus pyogenes
Tag	His tag
Molecular Weight	163 kDa
Concentration	10 mg/ml
Active temperature	This Cas9 is active at 37°C.
Formulation	Supplied as a solution of 30mM Tris-HCl, 0.3M NaCl, 50% Glycerol, 0.1 mM EDTA, pH 7.4
Storage & Stability	This product remains stable for up to 18 months at -20±5°C. Avoid repeated freeze-thaw cycles.