PEmax RNase deletion

PEmax RNase deletion involves modifications to the PEmax enzyme aimed at eliminating unwanted RNase activity, thus enhancing its stability and specificity. This advancement allows for more efficient target editing by minimizing the degradation of RNA components involved in the editing process, further improving the overall performance of the prime editing protocol.

产品编号	RC00003
规格	30 µg (3mg/ml) 100μg (10mg/ml) µg (10mg/ml) 500 μg (10mg/ml) 5 mg (10mg/ml) 大单询价
数量
价格	￥1050


联系我们

产品概述

Description

应用指导

single guide RNA (sgRNA) dependent single-stranded DNA cleavage

技术背景

References

David R Liu,et al.“Phage-assisted evolution and protein engineering yield compact, efficient prime editors”. Cell. 2023 Aug 31;186(18):3983-4002.e26.

质控参数

Purity	≥90% by SDS-PAGE, 98% by SEC-HPLC
Residual DNase	Non-specific DNase activity
Endotoxin Level	Control <10EU/mg

产品属性

Expression System	Recombinant Cas9-based fusion with three NLS expressed by E.coli
Species	S. pyogenes
Tag	C-His tag
Molecular Weight	223.714KDa
Storage & Stability	This product is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C.