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BsaI

BsaI product
RM00001
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Source E.coli that carries the cloned bsaIR gene from Geobacillus stearothermophilus.
Recognizes sites 5'--- G G T C T C (N)1 ---3'
3'--- C C A G A G (N)5---5'
Concentration 10 U/µL
Key Features · No star activity under suggested usage condition;
· Type IIS restriction enzymes target asymmetric DNA sequences and cleave outside their recognition sites;
· Accelerated digestion – Delivers precise digestion in 15 minutes;
· Animal free product.

Components
Components Size Storage
BsaI 1,000 U 5,000 U -20°C
10 × Reaction Buffer* (10 × Buffer1) 1 mL × 1 1 mL × 5 -20°C
Unit Definition One unit is defined as the amount of BsaI required to digest 1 µg of pUC57-GS DNA1 in 1 hour at 37°C in 50 µL of recommended reaction buffer.
1 pUC57-GS DNA is a plasmid that Genscript designed for restriction enzymes’ activity assay.
反应缓冲液 *The reaction buffer is intended for In vitro digestion assays of this product only, and not intended for other applications.
Storage & Stability Store at -20°C for up to 12 months from the date of manufacture. Avoid repeated freeze-thaw cycles. 

Research use only.



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