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BsmBI

BsmBI Product
RM00002
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Source E.coli that carries the cloned esp3IR gene from Bacillus stearothermophilu.
Recognizes sites 5'--- C G T C T C (N)1 ---3'
3'--- G C A G A G (N)5 ---5'
Concentration 10U/µL
Key Features ·Accelerated digestion – Delivers precise digestion in 15 minutes;
·Type IIS restriction enzymes target asymmetric DNA sequences and cleave outside their recognition sites;
·Optimal cleavage temperature is 55°C, with 70% activity preserved at 37°C;
·Animal free product.

Components
Components Size Storage
BsmBI 200 U 1,000 U -20 °C
10 × Reaction Buffer (10 × Buffer1) * 1 mL×1 1 mL×5 -20 °C
Unit Definition One unit is defined as the amount of BsmBI required to digest 1 µg of pUC-57-GS1 in 1 hour at 55°C in 50 µL of recommended reaction buffer. 
1 pUC57-GS DNA is a plasmid that Genscript designed for restriction enzymes’ activity assay.
反应缓冲液 * The reaction buffer is intended for In vitro digestion assays of this product only, and not intended for other applications.
Storage & Stability Store at -20 °C for up to 12 months from the date of manufacture. Avoid repeated freeze-thaw cycles. 

Research use only



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