目录产品 » GenCRISPR™/Cas9 基因编辑相关产品 » GenCRISPR™ Cas9 Enzymes » GenCRISPR™ SaCas9 2NLS Nuclease
GenCRISPR™ SaCas9 2NLS Nuclease

A 20 μl reaction in 1 × SaCas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and SaCas9 for 2 hours at 37 °C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

GenCRISPR™ SaCas9 2NLS Nuclease

GenCRISPR™ SaCas9 2NLS Nuclease is a tag free nuclease produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Staphylococcus aureus with a nuclear localization signal at both N-terminal and C-terminal.The small size of the nuclease facilitates enhanced in vivo delivery for genome editing in various organisms.
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Description

The GenCRISPR™ SaCas9 2NLS Nuclease can be formed with the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex to perform gene editing has been shown to reduce the challenges encountered with other CRISPR gene editing techniques such as viral and plasmid delivery. Challenges include off-target effects, cell viability and transcription/translational challenges. The SaCas9 recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at high efficiency with a variety of guide RNA (gRNA) spacer lengths.

GenCRISPR™ SaCas9 2NLS Nuclease is a tag free nuclease produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Staphylococcus aureus with a nuclear localization signal at both N-terminal and C-terminal.The small size of the nuclease facilitates enhanced in vivo delivery for genome editing in various organisms.
Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

产品概述
Key Features High knockout efficiencies: Consistent high performance in in-vitro plasmid cleavage test.
Tag-free: No extra tag amino acid.
DNA-free: No external DNA added to the system.

应用指导
gRNA-dependent double-stranded DNA cleavage

产品属性
Expression System Recombinant SaCas9 with an NLS at both N-terminal and C-terminal expressed by E.coli 
Species Staphylococcus aureus
Tag Tag-free
Molecular Weight ~130 kDa
Concentration 4 mg/ml
Active temperature This SaCas9 is active at 37°C.
Formulation Supplied as a solution of 20 mM Tris, 300 mM NaCl, 0.1 mM TCEP, 50% glycerol, pH 7.5
Storage & Stability This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles

质控参数
Appearance Clear, colorless liquid
Purity ≥ 90% as analyzed by SDS-PAGE
Concentration by A280 4 mg/ml±10%
Bioactivity (in vitro) ≥ 90%
Residual DNase Non-specific DNase activity
Residual RNase Non-specific RNase activity
Endotoxin Level ≤ 100 EU/mg as analyzed by gel clotting method

图例
  • GenCRISPR™ SaCas9 2NLS Nuclease
    • GenCRISPR™ SaCas9 2NLS Nuclease

    A 20 μl reaction in 1 × SaCas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and SaCas9 for 2 hours at 37 °C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.


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