| Product Description |
Glutathione
S-transferase (GST) Tag is engineered to create the GST gene fusion system, a
system used to purify and detect proteins of interest. The GST Tag is composed
of 220 amino acids, with a molecular weight of 26 kD and GST tagged proteins is
expressed in high levels in bacteria expression system and the fusion protein
is purified in a single step by affinity
chromatography using glutathione agarose. With site-specific proteases, such as
thrombin or factor Xa, the GST portion can easily be removed from GST tagged
protein. GST tag antibody is a universal tool for detection of GST tagged
protein.
GenScript GST Tag ELISA
Kit was
developed for three purposes, to identify GST tagged protein, monitoring the
protein expression and for screening the expression optimization of the protein.
It can also detect residual GST in the GST
tag cleavage during protein purification. However it is possible that improper folding of
GST or the presence of a fusion partner may prevent GST binding with the GST
monoclonal antibodies in this kit.
The kit detects
GST tagged protein in a sandwich immunoassay. It utilizes two clones of GST monoclonal
antibodies that bind to different epitopes of the GST tag protein at the same
time. The GST monoclonal antibody, pre-coated
on the plate, is used to capture GST tagged protein and the biotin conjugated GST
monoclonal antibody (Biotin-GST Mab) is added to interact with GST tagged
protein bound on the plate. Streptavidin-HRP is added to interact
with Biotin-GST Mab and the final step
involves the addition of TMB Solution that will react with HRP to give product
with an absorbance at 450 nm. The quantity of GST tagged protein in sample is
determined by comparisons with the absorbance of that of a known GST tagged
protein standard curve. |
| Key Features |
High Sensitivity: 1 ng/ml GST tagged protein
Broad Detection Range: 3.125~100 ng/ml
Application:
Identifying GST tagged protein
Checking GST tagged protein expression and screening the expression
optimization
Detecting residual GST in the GST
tag cleavage during protein purification
Rapid quantification
Feasible Operation:
Provide all reagents required for test
Complete the test in an hour |
| Precision |
Intra-assay Precision: CV<5%
Inter-assay Precision: CV<10% |
| Kit Contents |
| Components |
Quantity |
Part.No |
| GST Capture Plate |
1 plate (8 wells x 12 strips) |
411-80 |
| Sample Dilution Buffer |
30 ml |
411-60 |
| Biotin-GST Mab Stock |
200 µl |
411-20 |
| Antibody Dilution Buffer |
15 ml |
411-90 |
| Streptavidin-HRP |
12 ml |
411-30 |
| GST Protein Stock (1.0 mg/ml) |
10 µl |
411-10 |
| 20× Wash Solution |
40 ml |
B-A1-70 |
| TMB Solution |
12 ml |
B-A1-40 |
| Stop Solution |
6 ml |
B-A1-50 |
| Adhesive Plate Cover |
2 pieces |
N/A |
|
| Storage |
The unopened kit
is stable for at least 12 months if stored at 2-8°C and the opened kit may be stable
for up to 1 month at 2-8°C. Do not
freeze the kit. |
For research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.
