Protein A affinity chromatography resin is the most commonly applied method for the isolation and purification of IgG. Protein A is a cell wall component found in several strains of Staphylococcus aureus. It has five high affinity binding sites capable of binding specifically to the Fc region of immunoglobulin molecules from several species. Covalently immobilized Protein A matrices have been extensively used to purify IgG from several species of mammals.
GenScript's Monofinity A Resin is made of recombinant protein A as ligand, which not only keeps the specific binding capacity to Fc region of immunoglobulin molecules, but also enhances chemical stability under Cleaning-in-place (CIP) conditions. The typical dynamic binding capacity is more than 30 g human IgG/L settled resin. There is no significant decrease of binding capacity after purification/CIP for 200 cycles. In the meanwhile, the Monofinity A Resin has a low leakage of ligand (< 15 ng ligand/mg IgG) over a number of purification/CIP cycles.
As a high quality antibody purification resin, it is highly suitable for large scale antibody purification, and meets the purification requirements of industry-scale customers.
Product Details | Cat.No. | Product Name | Volume supplied | Resin content |
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| L00433-55 | Monofinity A Resin Prepacked Column (5mlx5) | 5 ml x 5 | Prepacked column | | L00433-51 | Monofinity A Resin Prepacked Column (5mlx1) | 5 ml x 1 | Prepacked column | | L00433-11 | Monofinity A Resin Prepacked Column (1mlx1) | 1 ml x 1 | Prepacked column | | L00433-15 | Monofinity A Resin Prepacked Column (1mlx5) | 1 ml x 5 | Prepacked column | | L00433-25 | Monofinity A Resin (25ml)
| 50 ml | 50% slurry | | L00433-200 | Monofinity A Resin (200ml) | 400 ml | 50% slurry | | L00433-1 | Monofinity A Resin (1L) | 2 L | 50% slurry | | L00433-5 | Monofinity A Resin (5L) | 10 L | 50% slurry | | L00433-10 | Monofinity A Resin (10L) | 20L | 50% slurry |
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