| Product Description |
CytoSinct CD4 Nanobeads, human is used for in
vitro enrichment of CD4+ cells from fresh or frozen peripheral
blood mononuclear cells (PBMCs), leukapheresis products, or single-cell
suspension based on the surface expression of human CD4.
To begin the isolation, Nanobeads are added to the cells. The
cells/beads mixed suspension is loaded onto a CytoSinct column placed in the
CytoSinct magnet, or other compatible columns with a magnet.
The CD4+ cells labeled with Nanobeads are retained
within the column and unlabelled cells (CD4- cells) are flushed out
during the wash step. After removing the column from the magnetic field, the
retained CD4+ cells can be eluted as the positively selected cell
fraction. |
| Format |
Supplied
in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and
Poloxamer 188. |
| Components |
1 mL or
0.5 mL CytoSinct CD4 Nanobeads, human Nanobeads conjugated to monoclonal
anti-human CD4 antibodies (isotype: mouse IgG1). |
| Volume |
Cat. No.
| Size | Capacity
|
|---|
| L00863-0.5 | 500 µL | Up to 5 × 108 total cells | | L00863-1 | 1 mL | Up to 1 × 109 total cells |
|
| Storage |
Store at 2 - 8 °C. Do not freeze.
|
CD4+ T cells were enriched from human PBMCs. CD4+ cells were stained with anti-CD4 (clone: SK3) APC and gated on Live/hCD45+.
For
research use only. Not intended for human or animal clinical trials,
therapeutic or diagnostic use.
