| Product Description |
CytoSinct
CD8 Nanobeads, human is used for in vitro enrichment of CD8+ cells from fresh or frozen
peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell
suspension based on the surface expression of human CD8.
To begin
the isolation, Nanobeads are added to the cells. The cells/beads mixed
suspension is loaded onto a CytoSinct column placed in the CytoSinct magnet, or
other compatible columns with a magnet.
The CD8+ cells labeled with
Nanobeads are retained within the column and unlabelled cells (CD8- cells) are flushed out
during the wash step. After removing the column from the magnetic field, the
retained CD8+ cells can
be eluted as the positively selected cell fraction. |
| Format |
Supplied
in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and
Poloxamer 188. |
| Components |
1 mL or
0.5 mL CytoSinct CD8 Nanobeads, human
Nanobeads conjugated to monoclonal
anti-human CD8 antibodies (isotype: mouse IgG1). |
| Volume |
| Cat. No. | Size | Capacity |
|---|
| L00864-0.5 | 500 µL | Up to 5 × 108 total cells | | L00864-1 | 1 mL | Up to 1 × 109 total cells |
|
| Storage |
Store at
2 - 8 °C. Do not freeze. |
CD8+ T cells were enriched from human PBMCs. CD8+ cells were stained with anti-CD8 (clone: SK1) BV650 and gated on Live/hCD45+.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
