| Product Description |
Streptavidin is
a protein that has similar binding properties to avidin. It is isolated from Streptomyces
Avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits.
Each subunit can bind one molecule of biotin. Biotin is a 244 dalton water-soluble
vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for
biotin.
Streptavidin-HRP
(M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly
sensitive detector conjugate binds to biotinylated primary or secondary
antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow
cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids
labeled with biotinylated nucleotide analogs in Southern or Northern blot. |
| Formulation |
Lyophilized
with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal |
| Reconstitution |
Reconstitute
the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final
concentration of 1 mg/ml. |
| Storage |
The product
remains stable in lyophilized form for at least 1 year if stored at 2-8°C,
-20°C, or lower from the date of receipt. For longer storage, -20°C or below is
recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or
for up to 1 year at -20°C or below. Avoid repeated freezing and thawing cycles.
If slight precipitation occurs
upon prolonged storage, clarify the solution by centrifugation before use. |
|
Working
concentrations for specific applications should be determined by titration
assay. Appropriate concentrations will be affected by several factors including
primary antibody, antigen amount, sensitivity of detection method, temperature,
and incubation time, etc. The following dilution ranges are just a guideline: |
| Application |
Recommended Usage |
| Dot blot |
1:2,000~1:10,000 |
| Western blot |
1:2,000~1:20,000 |
| ELISA |
1:5,000~1:20,000 |
| Others |
User optimized |
|
For research use only.
Not intended for human and animal therapeutic or diagnostic use.
