目录产品 » 稳定细胞系 » Human Recombinant H1 Histamine Receptor Stable Cell Line
HEK293/H1 Stable Cell Line

Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/H1 cells. The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to being stimulated with agonist Histamine. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Histamine (Mean ± SEM, n = 3). The EC50 of Histamine on this cell was 0.65 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

HEK293/H1 Stable Cell Line

Recombinant HEK293 cells stably overexpress human histamine receptor 1 (H1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00131
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产品概述
Product Description Recombinant HEK293 cells stably overexpress human histamine receptor 1 (H1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Applications Calcium mobilization assay, IP-One assay.

产品简介
Expressed Gene NCBI reference sequence NM_000861; no expressed tags
Target Protein NP_000852
Host Cell HEK293
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 50% DMEM (Cat. No. 10566-016, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium DMEM, 10% FBS
Culture Medium DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)

技术背景
Gene Synonyms HRH1, H1-R, H1R, HH1R, hisH1
Background The H1 histamine receptor is expressed primarily in the lungs, vasculature, and brain. The H1 mediates the contraction of smooth muscles, neurotransmission in the central nervous system, the release of catecholamine from adrenal medulla, and increases in capillary permeability due to contraction of terminal venules. H1's role in inflammatory responses makes its antagonist suitable for treating allergies.

图例
  • HEK293/H1 Stable Cell Line
    • HEK293/H1 Stable Cell Line

    Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/H1 cells. The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to being stimulated with agonist Histamine. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Histamine (Mean ± SEM, n = 3). The EC50 of Histamine on this cell was 0.65 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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