目录产品 » 稳定细胞系 » Human Recombinant BB3 Bombesin Receptor Stable Cell Line
CHO-K1/BB3 Stable Cell Line

Figure 1: Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB3 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB3 receptor agonist, Neuromedin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neuromedin B (Mean ± SEM, n = 3). The EC50 of Neuromedin B on this cell was 0.33 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/BB3 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human bombesin receptor subtype 3 (BB3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00183
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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human bombesin receptor subtype 3 (BB3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Applications Calcium mobilization assay.

产品简介
Expressed Gene NCBI reference sequence NM_001727; no expressed tags
Target Protein NP_001718
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. No. 10131-035, Gibco)

技术背景
Gene Synonyms BRS3; BB3R; BBR3
Background Bombesin, a bioactive peptide first identified in amphibian skin, is related to two mammalian peptides, gastrin-releasing peptide (GRP) and neuromedin B. A family of 4 GPCRs, including NMB-R (BB1), GRP-R (BB2), BRS-3 (BB3) and BRS-4, mediates the biological effects of the peptides. The bombesin receptor subtype 3, BB3 (also known as BRS3), is a third mammalian bombesin receptor subtype and no endogenous ligand has been identified to date. BB3 expression has been reported in lung (normal and cancer), nasal mucosa, placenta, and uterus. BB3-null mice have an obese phenotype, which suggests that BB3 may be an important target for obesity research. In addition, BB3 may be involved in diabetes and hypertension.

图例
  • CHO-K1/BB3 Stable Cell Line
    • CHO-K1/BB3 Stable Cell Line

    Figure 1: Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB3 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB3 receptor agonist, Neuromedin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neuromedin B (Mean ± SEM, n = 3). The EC50 of Neuromedin B on this cell was 0.33 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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