Figure 1: Ibutamoren -induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GHSR cells. The cells were loaded with Calcium-4 prior to being stimulated with a GHSR receptor agonist, Ibutamoren. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Ibutamoren (Mean ± SD, n = 2). The EC₅₀ of Ibutamoren on this cell was 14.28 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/GHSR Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human growth hormone secretagogue receptor (GHSR) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.

产品编号	M00189
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant CHO-K1 cells stably overexpress human growth hormone secretagogue receptor (GHSR) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 500 μg/ml G418 (Cat. No. 10131-035, Gibco)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Gibco), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1: Ibutamoren -induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GHSR cells. The cells were loaded with Calcium-4 prior to being stimulated with a GHSR receptor agonist, Ibutamoren. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Ibutamoren (Mean ± SD, n = 2). The EC₅₀ of Ibutamoren on this cell was 14.28 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

CHO-K1/GHSR Stable Cell Line

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