Figure 1: oxytocin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OXTR cells. The cells were loaded with Calcium-4 prior to stimulation with OXTR receptor agonist, oxytocin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of oxytocin (Mean ± SD, n = 2). The EC50 of oxytocin on CHO-K1/OXTR cells was 0.68 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/OXTR Stable Cell Line
M00195 | |
|
|
询价 | |
|
|
|
|
联系我们 |