Figure 1: Substance P-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK1 cells. The cells were loaded with Calcium-4 prior to stimulation with NK1 receptor agonist, Substance P. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Substance P (Mean ± SD, n = 3). The EC50 of Substance P on NK1/CHO-K1 cells was 67 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/NK1 Stable Cell Line
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