Figure 1: PACAP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PAC1 cells. The cells were loaded with Calcium-4 prior to being stimulated with PAC1 receptor agonist, PACAP. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PACAP (Mean ± SD, n = 2). The EC50 of PACAP on CHO-K1/PAC1 cells was 68.9 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/PAC1/Gα15 Stable Cell Line
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