目录产品 » 稳定细胞系 » Human Recombinant Corticotropin Releasing Factor Receptor CRF2 Stable Cell Line
CHO-K1/CRF2/Gα15 Stable Cell Line

Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with urocortin in CHO-K1/CRF2/Gα15 cells. d2 acceptor fluorophore -labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/CRF2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of urocortin on CHO-K1/CRF2/Gα15 cells was 0.086 nM.

CHO-K1/CRF2/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human corticotropin releasing hormone receptor 2 (CRF2) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
M00208
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human corticotropin releasing hormone receptor 2 (CRF2) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies), 200 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/CRF2/Gα15 Stable Cell Line
    • CHO-K1/CRF2/Gα15 Stable Cell Line

    Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with urocortin in CHO-K1/CRF2/Gα15 cells. d2 acceptor fluorophore -labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/CRF2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of urocortin on CHO-K1/CRF2/Gα15 cells was 0.086 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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