目录产品 » 稳定细胞系 » Human Recombinant GAL2 Galanin Receptor Stable Cell Line
CHO-K1/GALR2 Stable Cell Line

Figure 1: Galanin (1-30)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GALR2 cells. The cells were loaded with Calcium-4 prior to stimulation with a GALR2 receptor agonist, galanin (1-30). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of galanin (1-30) (Mean ± SEM, n = 3). The EC50 of galanin (1-30) on this cell was 0.37 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/GALR2 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human galanin receptor 2 (GALR2) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00209
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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human galanin receptor 2 (GALR2) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Applications Calcium mobilization assay.

产品简介
Expressed Gene NCBI reference sequence NM_003857; no expressed tags
Target Protein NP_003848
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. No. 10131-035, Gibco)

技术背景
Gene Synonyms GAL2-R, GALNR2, GALR-2
Background Actions of neuropeptide galanin are mediated by three G-protein coupled receptors, GalR1, GalR2, and GalR3. Human GALR2 was shown to couple to phospholipase C and to be involved in the elevation of intracellular calcium levels. GALR2 mRNA shows a wide distribution in the brain (mammillary nuclei, dentate gyrus, cingulate gyrus, posterior hypothalamic, supraoptic, and arcuate nuclei) and restricted peripheral tissue distribution with highest mRNA levels detected in human small intestine. Galanin has been implicated in anxiety-related behaviors, cognition, analgesia, and feeding in rodents.

图例
  • CHO-K1/GALR2 Stable Cell Line
    • CHO-K1/GALR2 Stable Cell Line

    Figure 1: Galanin (1-30)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GALR2 cells. The cells were loaded with Calcium-4 prior to stimulation with a GALR2 receptor agonist, galanin (1-30). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of galanin (1-30) (Mean ± SEM, n = 3). The EC50 of galanin (1-30) on this cell was 0.37 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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