Figure 1: Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHOK1/ADRA1A cells. The cells were loaded with Calcium-4 prior to being stimulated with an ADRA1A receptor agonist, epinephrine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of epinephrine (Mean ± SD, n = 2). The EC50 of epinephrine on this cell was 0.72 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/ADRA1A Stable Cell Line
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