目录产品 » 稳定细胞系 » Human Recombinant EP1 Prostanoid Receptor Stable Cell Line
HEK293/EP1 Stable Cell Line

Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/EP1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SEM, n = 3). The EC50 of PGE2 on this cell was 0.134 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

HEK293/EP1 Stable Cell Line

Recombinant HEK293 cells stably overexpress human prostaglandin E receptor 1 (PTGER1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00228
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产品概述
Product Description Recombinant HEK293 cells stably overexpress human prostaglandin E receptor 1 (PTGER1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Applications Calcium mobilization assay.

产品简介
Expressed Gene Codon optimized from NM_000955; no expressed tags
Target Protein NP_000946
Host Cell HEK293
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 50% DMEM (Cat. No. 10566, Gibco), 45% FBS (Cat. No. 10099-141, Gibco), 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium DMEM, 10% FBS
Culture Medium DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 250 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)

技术背景
Gene Synonyms PTGER1, EP1
Background Prostaglandins are known to affect the nervous system and can modulate synaptic transmission and neurotransmitter release, the sleep/wake cycle, fever, pain, and the immune system. Prostaglandin E2 receptor, EP1 subtype (EP1/PTGER1) is a receptor for prostaglandin E2 (PGE2). The members of the EP receptor family, EP1, EP2, EP3, and EP4, elicit their actions by altering cyclic adenosine monophosphate (cAMP) or intracellular calcium concentrations. EP1 activates phospholipase C and phosphatidylinositol turnover and stimulates the release of intracellular calcium via a Gi/Gq-coupled mechanism. EP2 and EP4 both signal through a Gs-coupled mechanism that stimulates adenylyl cyclase and increases intracellular levels of cAMP. EP1 appears to mediate the effects of PGE2 in promoting the formation of precancerous lesions in animal models of colon cancer. In addition, EP1 has an inhibitory effect on stress-induced aggressive and risk-taking behaviors in mice.

图例
  • HEK293/EP1 Stable Cell Line
    • HEK293/EP1 Stable Cell Line

    Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/EP1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SEM, n = 3). The EC50 of PGE2 on this cell was 0.134 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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