目录产品 » 稳定细胞系 » Human Recombinant EP1 Prostanoid Receptor Stable Cell Line
HEK293/EP1 Stable Cell Line

Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/EP1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SEM, n = 3). The EC50 of PGE2 on this cell was 0.134 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

HEK293/EP1 Stable Cell Line

Recombinant HEK293 cells stably overexpress human prostaglandin E receptor 1 (PTGER1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
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产品简介
Product Description Recombinant HEK293 cells stably overexpress human prostaglandin E receptor 1 (PTGER1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 250 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium DMEM, 10% FBS
Freeze Medium-DATA 50% DMEM (Cat. No. 10566, Gibco), 45% FBS (Cat. No. 10099-141, Gibco), 5% DMSO (Cat. No. D2650, Sigma)

图例
  • HEK293/EP1 Stable Cell Line
    • HEK293/EP1 Stable Cell Line

    Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/EP1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SEM, n = 3). The EC50 of PGE2 on this cell was 0.134 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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