Figure 1: Pancreatic Polypeptide-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPY4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with a NPY4 receptor agonist, pancreatic polypeptide. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of pancreatic polypeptide (Mean ± SD, n = 2). The EC50 of pancreatic polypeptide on this cell was 91 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom
CHO-K1/NPY4/Gα15 Stable Cell Line
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