Figure 1. ACTH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MC3/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with an MC3 receptor agonist, ACTH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ACTH (Mean ± SD, n = 2). The EC₅₀ of ACTH on this cell was 0.43 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/MC3/Gα15 Stable Cell Line

The melanocortin receptor 3, MC3 receptor, is Gs-coupled GPCRs expressed in brain, placental, and gut tissues but not in melanoma cells or in the adrenal gland. MC3 receptor knockouts exhibit a metabolic syndrome. At 4-6 months old, MC3 receptor knockout mice show increased fat mass, reduced lean mass and a higher feed efficiency, with normal metabolic rates. MC3 receptor knockout mice are hyperleptinaemic and males are often mildly hyperinsulinaemic.

产品编号	M00242
规格	2 vials 大单询价
数量
价格	询价


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Synonyms

MC3R, MC3, MC3-R, melanocortin 3 receptor, BMIQ9, OB20, OQTL

产品概述

Applications

Functional assay for MC3 receptor

产品简介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培养条件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

图例

Figure 1. ACTH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MC3/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with an MC3 receptor agonist, ACTH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ACTH (Mean ± SD, n = 2). The EC₅₀ of ACTH on this cell was 0.43 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/MC3/Gα15 Stable Cell Line

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