Figure 1. NECA-induced concentration-dependent stimulation of intracellular cAMP accumulation in CHO-K1/ADORA2A/Gα15 cells. The cells were stimulated with agonist NECA and the intracellular cAMP change was measured by Pherastar. The ration of fluorescence signals were normalized and plotted against the log of the cumulative doses of NECA (Mean ± SD, n = 2). The EC50 of NECA on this cell was 2.0 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/ADORA2A/Gα15 Stable Cell Line
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