目录产品 » 稳定细胞系 » Human Recombinant 5-HT2A Serotonin Receptor Stable Cell Line
CHO-K1/5-HT2A Stable Cell Line

Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2A cells. The cells were loaded with Calcium-4 prior to being stimulated with a 5-HT2A receptor agonist, 5-HT. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on this cell was 10.2 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/5-HT2A Stable Cell Line

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT2A which couples to Gq/11 is expressed throughout the central nervous system in the neocortex and olfactory tubercle. 5-HT2A receptor agonists may have important clinical value in the treatment of various disorders, such as depression, anxiety, bipolar disorder, and schizophrenia. GenScript's cloned human 5-HT2A–expressing cell line is generated in the CHO-K1 host.
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Description

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT2A which couples to Gq/11 is expressed throughout the central nervous system in the neocortex and olfactory tubercle. 5-HT2A receptor agonists may have important clinical value in the treatment of various disorders, such as depression, anxiety, bipolar disorder, and schizophrenia. GenScript's cloned human 5-HT2A–expressing cell line is generated in the CHO-K1 host.

Synonyms

5-HT2A receptor, 'D' receptor, HTR2, 5-HT2A, 5-hydroxytryptamine (serotonin) receptor 2A, 5Ht-2, 5-HT-2, 5-HT-2A, 5-hydroxytryptamine receptor 2A, serotonin 5HT-2 receptor, serotonin 5HT-2 receptor gene, serotonin receptor 2A, 5-HT2A receptor, Htr-2

Applications Functional assay for 5-HT2A receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

  • CHO-K1/5-HT2A Stable Cell Line
  • CHO-K1/5-HT2A Stable Cell Line

    Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2A cells. The cells were loaded with Calcium-4 prior to being stimulated with a 5-HT2A receptor agonist, 5-HT. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on this cell was 10.2 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


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