| Product Description |
Recombinant CHO-K1 cells stably overexpress human G protein subunit alpha 15 (Gα15) in the cytoplasm. This protein enables G protein-coupled receptor binding activity and acts upstream of or within with a positive effect on calcium-mediated signaling. |
| Culture Properties |
Adherent |
| Stability |
Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s), 10% FBS |
| Freeze Medium-DATA |
45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma) |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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