目录产品 » 稳定细胞系 » Human Recombinant Muscarinic Acetylcholine Receptor M2 Stable Cell Line
CHO-K1/M2/Gα15 Stable Cell Line

Figure 1. Oxotremorine M-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist oxotremorin M. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of oxotremorin M (Mean ± SEM, n = 3). The EC50 of oxotremorin M on this cell was 62.38 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/M2/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human cholinergic receptor muscarinic 2 (M2) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00258
询价

联系我们
产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human cholinergic receptor muscarinic 2 (M2) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Applications Binding assay, calcium mobilization assay, and cAMP accumulation assay.

产品简介
Expressed Gene NCBI reference sequence NM_000739; no expressed tags
Target Protein NP_000730
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)

技术背景
Gene Synonyms M2R, HM2, CHRM2
Background Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M2 muscarinic receptor couples to Gi/o to inhibit cAMP production. GenScript co-transfected human M2 with Gα15 in the CHO-K1 which supports high levels of recombinant M2 expression on the cell surface and contains high levels of Gα15 to couple the receptor to the calcium signaling pathway.

图例
  • CHO-K1/M2/Gα15 Stable Cell Line
    • CHO-K1/M2/Gα15 Stable Cell Line

    Figure 1. Oxotremorine M-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist oxotremorin M. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of oxotremorin M (Mean ± SEM, n = 3). The EC50 of oxotremorin M on this cell was 62.38 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


喜欢新升级的网站吗？

讨厌

不喜欢

一般

喜欢

非常喜欢

*
Skip