目录产品 » 稳定细胞系 » Human Recombinant Muscarinic Acetylcholine Receptor M3 Stable Cell Line
CHO-K1/M3 Stable Cell Line

Figure 1. Carbacol-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M3 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist Carbacol. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Carbacol (Mean ± SEM, n = 3). The EC50 of Carbacol on this cell was 13.32 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/M3 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human cholinergic receptor muscarinic 3 (M3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00259
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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human cholinergic receptor muscarinic 3 (M3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Applications Binding assay, Calcium flux assay, IP-One assay

产品简介
Expressed Gene NCBI reference sequence NM_000740; no expressed tags
Target Protein NP_000731
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)

技术背景
Gene Synonyms CHRM3, EGBRS, HM3, PBS, m3AChR
Background Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M3 muscarinic receptors are located at many places in the body, e.g. smooth muscles, endocrine, exocrine glands, as well as lungs. They are also found in the CNS, where it induces emesis. They generally cause smooth muscle contraction and increased glandular secretions.

图例
  • CHO-K1/M3 Stable Cell Line
    • CHO-K1/M3 Stable Cell Line

    Figure 1. Carbacol-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M3 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist Carbacol. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Carbacol (Mean ± SEM, n = 3). The EC50 of Carbacol on this cell was 13.32 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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