Figure 1. Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA1B cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist, Epinephrine. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Epinephrine (Mean ± SD, n = 2). The EC50 of Epinephrine on this cell was 17.7 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/ADRA1B Stable Cell Line
M00260 | |
|
|
询价 | |
|
|
|
|
联系我们 |