目录产品 » 稳定细胞系 » Human Recombinant P2Y12 Purinergic Receptor Stable Cell Line
1321N1/P2Y12/Gα15 Stable Cell Line

Figure 1: ADP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y12/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a P2Y12 receptor agonist, ADP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ADP (Mean ± SEM, n = 3). The EC50 of ADP on 1321N1/P2Y12/Gα15 cells was 78.85 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

1321N1/P2Y12/Gα15 Stable Cell Line

Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y12 (P2Y12) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00261
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产品概述
Product Description Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y12 (P2Y12) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Applications Calcium mobilization assay, IP-One assay.

产品简介
Expressed Gene NCBI reference sequence NM_022788; no expressed tags
Target Protein NP_073625
Host Cell 1321N1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% DMEM (Cat. No. 10569, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium DMEM, 10% FBS
Culture Medium DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 50 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)

技术背景
Gene Synonyms P2RY12, ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12, SP1999
Background The P2Y receptor 12 (P2Y12) is a Gi-coupled GPCR expressed in platelets, brain tissues, vascular smooth muscle cells. P2Y12 can sustain platelet aggregation and promote thrombus growth and stabilization. It also plays a function in dense and alpha granule secretion, p-selectin expression and microparticle formation GenScript’s human P2Y12-expressing stable cell is guaranteed to function properly in calcium flux assay.

图例
  • 1321N1/P2Y12/Gα15 Stable Cell Line
    • 1321N1/P2Y12/Gα15 Stable Cell Line

    Figure 1: ADP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y12/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a P2Y12 receptor agonist, ADP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ADP (Mean ± SEM, n = 3). The EC50 of ADP on 1321N1/P2Y12/Gα15 cells was 78.85 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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